A common Protein Extraction Protocol for Proteomic Analysis: Horse Gram a Case Study
- 1 Biotechnology Division, CSIR-IHBT, Palampur-176061 (HP), India
Abstract
Three protocols viz. Trichloroacetic Acid (TCA)-acetone, phenol and multi-detergent were compared for the extraction of proteins from horsegram (Macrotyloma uniflorum) which is less studied yet nutritionally and economically very important pulse crop. Almost equal quantity of proteins was extracted by all the three protocols. However, quality of protein from phenol method was superior to that from other protocols. Bands in One-Dimensional (1-D) and Two-Dimensional Electrophoresis (2-DE) of phenol protocol were reproducible and better. We report here a modified and common protocol for the extraction of proteins for proteomic analysis. The developed protocol is applicable not only to other economically important leguminous but also non-leguminous crops.
DOI: https://doi.org/10.3844/ajabssp.2013.293.301
Copyright: © 2013 Jyoti Bhardwaj and Sudesh Kumar Yadav. This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Trichloroacetic Acid (TCA)
- Pinch of Bromophenol Blue (PBB)
- Two-Dimensional Electrophoresis (2-DE)
- One-Dimensional (1-D)
- Sodium Dodecyl Sufate (SDS)