Enzymatic Kinetics of Fatty Acids Methyl Ester from Kapok Seed Oil (Ceiba Petandra) Using Lipozyme TL IM

Abstract

Synthesis of Fatty Acids Methyl Ester (FAME) utilizing oil extracted from kapok seeds (Ceiba petandra) and catalyzed by lipozyme TL. 1M via enzymatic kinetics transesterification was succesfully conducted A mixture of different FAME, widely known as biodiesel is one of the sources of renewable energy. A kinetic analysis was investigated to identify the reaction model and the rate equation and to calculate kinetic parameters. The result obtained showed that the enzymatic kinetics followed a Michaelis-Menten model. The transesterification kinetic followed the Ping-pong Bi-Bi mechanism characterized by the υ_max, K_m(KO), K_m(Met) values of 3.06×10^-5 mmol/min.mg catalyst, 0.95×10² ppm, 1.30×10² ppm, respectively.