Phylogenetic of ERIC-DNA Fingerprinting and New Sequencing of Aeromonas Species and V. Cholerae DNA

Abstract

The current study included 44 isolate of A. hydrophila, A. sobria and V. cholerae and other bacteria isolated from stool samples and environmental samples (Kufa river water and hospital environmental samples). ERIC DNA Fingerprinting with ERIC primers pairs generated distinct amplification bands ranging in size from (87 bp to 8000 kb). The 44 isolates produced 93 different patterns by ERIC DNA fingerprinting. The fingerprinting patterns of the isolates were constructed using cluster analysis the UPGMA (group method) using average linkages Number of different bands (Similarity coefficient), 1% tolerance. The PCR method of gene (16SrDNA and 16SrRNA) were the best methods for diagnosis, which has led to isolate and diagnosis of A. hydrophila A. sobria and V. cholerae are distributed as clinical isolates of A. hydrophila A. sobria were diarrhea samples. While, the environmental isolates were isolate of V. cholerae from Kufa river water. Sequencing technology is used to diagnosis of A. hydrophila, A. sobria and V. cholerae isolates were examined by (16SrDNA, 16SrRNA) genes. Recorded the new isolates in Nucleotide/Blast and recorded as the first sequencing in Gene-Bank/NCBI, DDBJ and ENA (INSDC). Each sequence have Accession number (No.: Gene bank: LC194875 Aeromonas sobria-HNK1, Gene bank: LC194876 Aeromonas hydrophila-HNK2, Gene bank: LC194877 Vibrio cholerae-HNK3) this is the first study in Iraq for discovery of new isolates by new sequences. The frequency of A. hydrophila, A. sobria and V. cholerae isolates in Najaf were higher among clinical and environmental isolates. The ERIC band pattern is an adequate tool for epidemiological investigations of A. hydrophila, A. sobria and V. cholerae isolates.