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Frequency: Quarterly
ISSN: 1608-4217 (Print)
ISSN: 2410-8561 (Online)
Investigation Open Access

Development of Genetic Constructions for Exctocytosis Control

Irina Nikolaevna Dominova1, Vitaly Anvarovich Kasymov1, Ekaterina Vladimirovna Silina2, Victor Aleksandrovich Stupin3, Natalia Nikolaevna Shusharina1 and Maksim Vladimirovich Patrushev1
  • 1 Immanuel Kant Baltic Federal University (IKBFU) Nevskogo Str., 14, Kaliningrad, 236041, Russia
  • 2 I.M. Sechenov First Moscow State Medical University (Sechenov University) Trubetskaya Str, 8, Moscow, 119991, Russia
  • 3 N.I. Pirogov Russian National Research Medical University (RNRMU) Ostrovityanova St., 1, Moscow, 117997, Russia

Abstract

A fragment of the research project devoted to the development of genetic control of exocytosis is presented in the work, which can further ensure the success of targeted therapy of various diseases, including neurodegenerative ones. For the purpose of specific transfection of intracellular cascades in astrocytes in vivo, we have developed an experimental sample of the reagent kit. The latter represents lentiviral genetic construction LVV-GFAP-Case12. The results of testing of experimental samples of the reagent kit for specific transfection of astroglial cells are presented with the purpose of targeted control of intracellular cascades in vivo on acute slices of different parts of the brain in adult Wistar rats. We selected regions of interest with cells expressing calcium indicator Case12 and responsible for ATP application by >2-fold amplification of fluorescence. We have developed instructions for using the reagent kit for specific transfection of astroglial cells with the purpose of targeted control of intracellular cascades.

OnLine Journal of Biological Sciences
Volume 18 No. 2, 2018, 162-168

DOI: https://doi.org/10.3844/ojbsci.2018.162.168

Submitted On: 21 September 2017 Published On: 29 January 2018

How to Cite: Dominova, I. N., Kasymov, V. A., Silina, E. V., Stupin, V. A., Shusharina, N. N. & Patrushev, M. V. (2018). Development of Genetic Constructions for Exctocytosis Control. OnLine Journal of Biological Sciences, 18(2), 162-168. https://doi.org/10.3844/ojbsci.2018.162.168

Open AccessCopyright: © 2018 Irina Nikolaevna Dominova, Vitaly Anvarovich Kasymov, Ekaterina Vladimirovna Silina, Victor Aleksandrovich Stupin, Natalia Nikolaevna Shusharina and Maksim Vladimirovich Patrushev. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Astrocytes
  • Astroglia
  • Exocytosis
  • Genetic Structures
  • Reagents
  • Transfection
  • Targeted Control